CRISPR-EZ Cas9 electroporated genetic modification order form CRISPR-EZ Cas9 electroporated genetic modification order form Contact Information PI name * PI email PI phone Contact person Contact email Contact phone Project Information Name of genes Types of targeting Knockout (non-homologous end joining) Knock In (homologous donor repair by ssDNA) # of sgRNAs in 1 electroporation Region targeted Precise genomic coordinates gRNAs Sequence of gRNAs Site of insertion (if knock in) Size and sequence of ssDNA (if applicable) Expected outcome for genetic modification Are pre-weaning phenotypes expected? Yes No If yes, explain. Mouse protocol # Service summary * USC client: CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene targeting 80 zygotes (B6D2F1 strain)—knockout Other academic institution client: CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene targeting 80 zygotes (B6D2F1 strain)—knockout Industry client: CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene targeting 80 zygotes (B6D2F1 strain)—knockout USC client: CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene editing using single strand DNA donor (up to 100 bp) targeting 100-120 zygotes (B6D2F1 strain)—knock-in Other academic institution client: CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene editing using single strand DNA donor (up to 100 bp) targeting 100-120 zygotes (B6D2F1 strain)—knock-in Industry client: CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene editing using single strand DNA donor (up to 100 bp) targeting 100-120 zygotes (B6D2F1 strain)—knock-in Additional project information helpful for performing the job Billing Information PPGG # Cost center Are you a non-USC client (i.e., other academic institution, industry)? Yes No PI signature * Date * PIs will be responsible for supplying sgRNA and, if applicable, ssDNA. The Genome Modification Facility will provide ordering information for best sources. After this form is submitted, the Genome Modification Facility will assess the job, provide a job number, schedule a date for the procedure, and notify the PI and the contact person. The outcomes for all procedures are dependent on a variety of factors, many of which are beyond the control of the facility. Whereas the facility has had excellent outcomes over many years for all procedures offered, outcomes of individual projects cannot be guaranteed. The investigator agrees to pay for the services, independent of the outcome. If you are human, leave this field blank. This form is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply. Submit
