The outcomes for all procedures are dependent on a variety of factors, many of which are beyond the control of the facility. Whereas the facility has had excellent outcomes over many years for all procedures offered, success cannot be guaranteed.
*Prices are currently being re-evaluated and are subject to change.
| Service | Order Form | USC | Other Academic Institutions | Non-Academic Clients |
| 1. CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene targeting 80 zygotes (B6D2F1 strain)—knockout | CRISPR-EZ Cas9 electroporated genetic modification | $2,800 | $3,640 | $5,600 |
| 2. CRISPR-EZ Cas9 electroporated genetic modification: gRNA/CAS9 gene editing using single strand DNA donor (up to 100 bp) targeting 100-120 zygotes (B6D2F1 strain)—knock-in | CRISPR-EZ Cas9 electroporated genetic modification | $3,500 (excluding cost of gRNAs and ssDNA template) | $4,550 (excluding cost of gRNAs and ssDNA template) | $7,000 (excluding cost of gRNAs and ssDNA template) |
| 3. Standard zygote DNA injection: random integration transgenic mouse production targeting 200 zygotes (B6D2F1 strain) | Zygote DNA injection | $4,600 (DNA template prepared by investigator – protocol provided) | $5,980 (DNA template prepared by investigator – protocol provided) | $9,200 (DNA template prepared by investigator – protocol provided) |
| 4. Zygote DNA injection: directed gRNA/CAS9 gene editing using double stranded DNA template in homologous donor repair targeting 200 zygotes (B6D2F1 strain) | Zygote DNA injection | $4,800 (excluding cost of gRNAS and template prepared by investigator – protocol provided) | $6,240 (excluding cost of gRNAS and template prepared by investigator – protocol provided) | $9,600 (excluding cost of gRNAS and template prepared by investigator – protocol provided) |
| 5. Zygote DNA injection: gRNA/CAS9 directed insertion into H11 landing pad (see PMID: 32169219) targeting 200 zygotes | Zygote DNA injection | $5,200 (DNA template vector supplied by GMF, DNA template prepared by investigator protocol provided, gRNA cost included) | $6,760 (DNA template vector supplied by GMF, DNA template prepared by investigator protocol provided, gRNA cost included) | $10,400 (DNA template vector supplied by GMF, DNA template prepared by investigator protocol provided, gRNA cost included) |
| 6. Mouse sperm cryopreservation from 2 procedure compatible males | Mouse sperm cryopreservation and validation | $1,000 (without secondary in vitro fertilization test of cryopreserved sperm) | $1,300 (without secondary in vitro fertilization test of cryopreserved sperm) | $2,000 (without secondary in vitro fertilization test of cryopreserved sperm) |
| 7. In vitro fertilization validation of cryopreserved sperm – 50 donor eggs (B6D2F1 strain) | Mouse sperm cryopreservation and validation | $600 | $780 | $1,200 |
| 8. Strain derivation through frozen sperm, in vitro fertilization (150 B6D2F1 strain donor eggs), and embryo transfer | Strain derivation through frozen sperm and in vitro fertilization | $2,600 | $3,380 | $5,200 |
| 9. Sperm cryobanking |
