Zygote DNA injection order form Zygote DNA injection order form We will harvest B6D2F1 zygotes and inject into the pronucleus of fertilized ova with DNA prepared and purified by the investigator (following protocols provided by the Genome Modification Facility). Investigators will need to supply a photo of the gel of the purified DNA for injection, and details of the concentration of DNA. Injected eggs that survive to the 2 cell stage will be implanted into pseudopregnant females. Contact Information PI name * PI name First First Last Last PI phone PI email Contact name Contact name First First Last Last Contact email Contact phone Address Project Information Name of injection construct Size of transgene fragment (kb) Detailed sequence map of DNA to be injected Product * USC client: Standard zygote DNA injection—random integration transgenic mouse production targeting 200 zygotes (B6D2F1 strain) Other academic institution client: Standard zygote DNA injection—random integration transgenic mouse production targeting 200 zygotes (B6D2F1 strain) Industry client: Standard zygote DNA injection—random integration transgenic mouse production targeting 200 zygotes (B6D2F1 strain) USC client: Zygote DNA injection—directed gRNA/CAS9 gene editing using double stranded DNA template in homologous donor repair targeting 200 zygotes (B6D2F1 strain) Other academic institution client: Zygote DNA injection—directed gRNA/CAS9 gene editing using double stranded DNA template in homologous donor repair targeting 200 zygotes (B6D2F1 strain) Industry client: Zygote DNA injection—directed gRNA/CAS9 gene editing using double stranded DNA template in homologous donor repair targeting 200 zygotes (B6D2F1 strain) USC client: Zygote DNA injection—gRNA/CAS9 directed insertion into H11 landing pad (see PMID: 32169219) targeting 200 zygotes Other academic institution client: Zygote DNA injection—gRNA/CAS9 directed insertion into H11 landing pad (see PMID: 32169219) targeting 200 zygotes Industry client: Zygote DNA injection—gRNA/CAS9 directed insertion into H11 landing pad (see PMID: 32169219) targeting 200 zygotes Are pre-weaning phenotypes expected? yes no If yes, explain. Mouse protocol # Additional project information helpful for performing the job Billing Information PPGG # Cost center Are you a non-USC client (i.e., other academic institution, industry)? Yes No Date * PI signature * After this form is submitted, the Genome Modification Facility will assess the job, provide a job number, schedule a date for the procedure, and notify the PI and the contact person. The outcomes for all procedures are dependent on a variety of factors, many of which are beyond the control of the facility. Whereas the facility has had excellent outcomes over many years for all procedures offered, outcomes of individual projects cannot be guaranteed. The investigator agrees to pay for the services, independent of the outcome. If you are human, leave this field blank. This form is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply. Submit Start Over
